Immune Mimicry In Malaria Tctp Oetken

Endotoxin Removal-Since the TCTP was grown through the reproduction of E.coli, the E.coli needed to be killed in order to retrieve the TCTP, however, this left bits of LPS all over, so they needed to be removed in order to have pure TCTP in order for the study to be effective. So, they introduced lumulus amoebocytes before the removal of endotoxins and after (the amoebocytes release chemicals when they encounter LPS, so it is basically a measure of how much LPS is in the system). They removed the LPS with Detoxi Gel agar which results in a 1000 fold reduction in endotoxins.

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