Melissa's Paper

For subtractive immunization, monocolonal antibodies are produced. In this study they were first injected mice with Mamu-AG periodically for several weeks. This created memory-T-cells against Mamu-AG, but they could also be against Mamu-A since they have similar structures. Next, they injected mice with Mamu-A and cyclophosphamide (which kills proliferating cells especially proliferating immune cells). In this cause cyclophosphamide killed all the T-cells cells that were specific for Mamu-A but not those that were specific for only Mamu-AG. These T-cell were not proliferating when cyclophosphamide was present. Then, they cut open the mice lypmphocytes and used flow cytometry & ELISA was used to select the specific Mamu-AG antibodies.