Polyclonal Vs Monoclonal

In order to obtain polyclonal antibodies
1. Inject a particular antigen into a mouse in which you want the antibodies to that particular antigen.
2. 2 weeks later, extract blood from the mouse and centrifuge it so that the serum w/the particular antibody.
*Note: The antibody in the serum is against the particular antigen that may be receptive to many epitotes
*The polyclonal antibodies come from a mixed population of B-Cells
In order to obtain monoclonal antibodies
1. Inject a particular antigen into a mouse inwhich you want the antibodies to that particular antigen.
2. After 2 days, take the mouses spleen out and purify the B-cells in it. Get a single B-cell with the right immunoglobin against the particular antigen
3. Culture the single B-cell for clonal expansion. The B-cells differentiate into plasma cells that secrete the antibody against the particular antigen receptive to only 1 epitote.
4. The antibody is purified from the cultured fluid from the cultured B-Cells

Main Difference-For monoclonal antibodies, they are all against a single epitote b.c they came from a homogenous population of B-cells, not heterogenous like polyclonal.

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