Willie's Paper

Willie’s Presentation- Specific Role of mitochondrial electron transport in blood-stage Plasmodium falciparum

Background- P falciparum mitochondria only
Main Point- test if the acquisition of DHOD could render mitochondrial

Methods- Introduced a plasmid containing yeast DHOD and a gene encoding GFP
-Location of DHOD-GFP gene was determined by immunofluorescence

Graphs- b1- Northern blot just shows that the gene was successfully implemented into the genome
Figure 2- growth is inhibited in wildtype parasite by atovoquanone but does not effect transgenic cells
Mito tracker- shows where there is a electrical difference in membrane of mitochondria

Results- Atovaquone inhibits wild type but not transgenic parasites
-Using both atovaquone and proguanil combination works best to collapse mt membrane potential
-P falciparum can acquire independence

What does DHOD do? Helps complete the final step of the accepting of electrons in the mitochondria. They also grow better in proguanil than wild type parasites

Here is a link to a good Video of the malarial life cycle. Click on the link underneath the picture of the mosquito to view the movie.

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